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Mixed activation and mixed inhibition of the oxidoreductase activity of Rhus vernicifera laccase by different Pd 2+ ion concentration
Author(s) -
Tu ChuQiao,
Liang Hong,
Wang GuangHui
Publication year - 1999
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.19990170504
Subject(s) - chemistry , laccase , non competitive inhibition , catalysis , ion , reaction rate constant , kinetics , enzyme , nuclear chemistry , inorganic chemistry , stereochemistry , organic chemistry , physics , quantum mechanics
Kinetic experiments were performed to study the effects of Pd 2+ ion on the oxidation of 5,6‐dibromo‐2, 3‐dicyanohydroquinone catalyzed by Rhus vernicifera laccase under condition of pH 4.5 and 30 ± 0.1°C. The results showed that the mixed activation could be observed when Pd 2 ion was at low concentrations. The competitive and non‐competitive activation constants were 9 × 10 − ‐ 6 and 2 × 10 − ‐ 6 mol/L, respectively. With the increase of Pd 2+ ion concentration, the activation was gradually converted into mixed inhibition, and the competitive and non‐competitive inhibition constants were 6 × 10 − ‐ 6 and 32 × 10 − ‐ 6 mol/L, respectively.