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N‐(β‐Carboxypropionyl)luminol as a new chemiluminescence label in immunoassay
Author(s) -
Zhuang HuiSheng,
Wang QiongE,
Zhang Fan,
Huang QiaoJia,
Tang YuChai
Publication year - 1997
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.19970150206
Subject(s) - chemistry , luminol , chemiluminescence , chemiluminescent immunoassay , immunoassay , chromatography , molar ratio , mole , nuclear chemistry , antibody , biochemistry , immunology , catalysis , biology
A new chemiluminescence label N ‐(β‐carboxpropionyl)luminol (CPL) was used to label sheep anti‐human IgG (SaHIgG). The labeled antibody waa stable and could be detected at least dawn to 10 − 17 ∼10 − 16 mol. The molar incorporation ratio was estimated to be 0.26 mol of CPL per mol of SaHIgG. There were no apparent changes in the immunoreactivity of the labeled SaHIgG and in the quantum efficiency of the CPL after labeling.