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A sensitive xanthine oxidase electrode with silk net for estimating fish freshness
Author(s) -
TuZhi Peng,
LiJu Yang,
LianQing Shen
Publication year - 1996
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.19960140505
Subject(s) - chemistry , xanthine oxidase , electrode , hydrogen peroxide , hypoxanthine , detection limit , immobilized enzyme , biosensor , fish <actinopterygii> , platinum , clark electrode , chromatography , xanthine , nuclear chemistry , enzyme , fishery , biochemistry , catalysis , electrolyte , biology
An enzyme biosensor was constructed using a plate platinum electrode and immobilized xanthine oxidase (XOD). Only a very small quantity of enzyme was chemically immobilized on a special silk net. Hydrogen peroxide released during the enzymatic reaction was detected by the electrode at +0.65 V (vs. Ag/AgCl). The electrode was very sensitive to hypoxanthine and its detection limit was 1×10– 7 mol/L. When it was applied to the determination of fish freshness, the results agreed well with those obtained by traditional methods‐determination of total volatile basic nitrogen (TVB‐N) and microbial count. A range for estimating the freshness of river fish was suggested.