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Methylene green‐mediated sensor highly sensitive to hydrogen peroxide based on entrapment of horseradish peroxidase in composite membrane of regenerated silk fibroin and poly (vinyl alcohol)
Author(s) -
XinXin Wu,
HaiYing Liu,
DeYao Qi,
YongCheng Liu,
JiangHong Qian,
ShengHui Liu,
TongYin Yu,
JiaQi Deng
Publication year - 1996
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.19960140413
Subject(s) - fibroin , chemistry , horseradish peroxidase , hydrogen peroxide , vinyl alcohol , amperometry , membrane , detection limit , cyclic voltammetry , methylene blue , composite number , chemical engineering , polymer chemistry , electrochemistry , silk , chromatography , electrode , organic chemistry , polymer , catalysis , composite material , materials science , biochemistry , photocatalysis , enzyme , engineering
A novel composite membrane of poly(vinyl alcohol) and regenerated silk fibroin was employed to immobilize horseradish peroxidase (HRP) and IR was used to give a useful insight into the structure of the composite membrane before and after ethanol treatment. A methylene green‐mediated sensor sensitive to hydrogen peroxide was fabricated, based on the composite membrane as immobilization matrix for HRP. Cyclic voltammetry and amperometric measurement were for the first time utilized to demonstrate the suitability of methylene green as an electron transfer mediator between immobilized HRP and a glassy carbon electrode in bioelectrocatalytic reduction of hydrogen peroxide. Performance and characteristics of the sensor were evaluated in respect to response time, detection limit, applied potential and concentration of the mediator. The sensor possesses a variety of characteristics including good sensitivity, rapid response time and low detection of limit of 0.1 μmol/L.