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Electrochemically modified piezoelectric crystal biosensor for detection of Staphylococcus aureus
Author(s) -
ShiHui Si,
JinHua Chen,
FengJiao He,
LiHua Nie,
ShouZhuo Yao
Publication year - 1996
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.19960140307
Subject(s) - chemistry , staphylococcus aureus , biosensor , piezoelectricity , crystal (programming language) , piezoelectric sensor , calibration , calibration curve , detection limit , chromatography , composite material , biochemistry , materials science , bacteria , genetics , computer science , biology , programming language , statistics , mathematics
Electropolymerized o ‐phenylenediamine film is used as a functional coating for the immobilization of anti‐ S. aureus antibody on the surface of a gold‐plated piezoelectric crystal, and this piezoelectric immunosensor is applied to detect S. aureus. The frequency shift (Δ F = F20z ‐ F 380z, Hz) between the frequency at the 20th second (after the addition of sample, F 20Z) and that at 380 seconds later (F380z) was introduced to construct a calibration graph, and shortening of assay time was achieved. The S. aureus concentrations in the range of 10 5– 10 9 cells/mL can be detected by this system.