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Large scale synthesis of oligoribonucleotides on PEG by the phosphite triester approach
Author(s) -
Xu ZuSheng,
Yang ZaiWan,
Wang Yu
Publication year - 1995
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.19950130309
Subject(s) - chemistry , phosphoramidite , ribonucleoside , yield (engineering) , peg ratio , combinatorial chemistry , dodecameric protein , organic chemistry , biochemistry , dna , rna , oligonucleotide , molecule , materials science , finance , economics , metallurgy , gene
Large scale synthesis of oligoribonucleotides has been successfully performed on PEG support by the phosphoramidite approach using t ‐butyldimethylsilyl to protect the 2'‐hydroxyl group of ribonucleoside. By means of this procedure, the dodecamer r(AGUGGUCUUUGU) was synthesized in 98.1% average coupling yield, and 55 mg pure product was obtained from one gram of functionalized PEG.