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Surface acoustic wave sensor system applied to the kinetic study of urease in plant seeds
Author(s) -
Liu DeZhong,
Chen Kang,
Ge Kai,
Me LiHua,
Yao ShouZhuo
Publication year - 1995
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.19950130307
Subject(s) - chemistry , urease , urea , kinetic energy , surface acoustic wave , surface (topology) , analytical chemistry (journal) , chromatography , biological system , acoustics , biochemistry , physics , quantum mechanics , biology , geometry , mathematics
A new type of the surface acoustic wave (SAW) sensor system was delivered. Urease from several kinds of plant seeds was extracted with different extracting solvents. The urease activity, Michaelis constant and other kinetic parameters were estimated for the first time by means of the new device–‐SAW sensor system. Some factors such as pH, temperature, activators and inhibitors are also discussed. The method can be applied to the determination of urea content in human urine and the experimental results consist with those reported.