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Structure effect of nucleotides in terbium(III)—nucleotide fluorescent reaction New evidence for the binding sites of terbium(III) on nucleotides
Author(s) -
Li YuanZong,
Chang WenBao,
Ci YunXiang
Publication year - 1993
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.19930110606
Subject(s) - chemistry , nucleotide , gtp' , terbium , guanine , fluorescence , stereochemistry , biochemistry , organic chemistry , ion , enzyme , physics , quantum mechanics , gene
Upon addition of Tb 3+ to 16 nucleotides and homopolynucleotides, all of them showed a characteristic green emission from Tb 3+ , but with much different intensity, upon excitation in the aromatic region of bases. The result suggested that nucleotides with at least one carbonyl group in nucleotide bases are better enhancers to the fluorescence of Tb 3+ . The complexes of ATP, GDP and GTP with Tb 3+ are synthesized as two types of models. Guanine type nucleotides with one carbonyl group in the bases are the best enhancers, while adenine type nucleotides with no carbonyl group in the bases are poorest enhancers to the fluorescence of Tb 3+ . Comparing the IR spectra of ATP, GTP, GDP and their Tb 3+ complexes suggested that C‐6 carbonyl group in GTP and GDP may be involved in complex formation, which may be responsible for the effective energy transfer. This is further supported by comparing the UV spectra of ATP, Poly(A), GTP, and Poly(G) with their Tb 3+ complexes in water solution.