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Computer‐assisted optimization for the selection of mobile phase in semi‐preparative HPLC
Author(s) -
Wang QinSun,
Gao RuYu,
Yan BingWen
Publication year - 1993
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.19930110111
Subject(s) - chemistry , selection (genetic algorithm) , high performance liquid chromatography , chromatography , phase (matter) , computer science , artificial intelligence , organic chemistry
A computer‐assisted method is presented for optimization for the selection of mobile phase composition in semi‐preparative HPLC. The optimization for the expected separation is based on a polynomial estimation from five preliminary runs. Statistical scanning technique was used for optimization. Double criteria simulation system (DCSS) is established for chromatographic performance measurement in this method. The validity of the optimization strategy is confirmed by applying it to a technical Cypermethrin separation. Excellent agreement is obtained between the predicted and experimental results.