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Optimization of two‐factor (pH and ion concentration) simultaneous selection in HPLC using a statistical method
Author(s) -
Wang QinSun,
Gao RuYu,
Yan BingWen
Publication year - 1992
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.19920100207
Subject(s) - chemistry , selection (genetic algorithm) , dimension (graph theory) , chromatography , high performance liquid chromatography , resolution (logic) , ion , analytical chemistry (journal) , biological system , artificial intelligence , computer science , mathematics , organic chemistry , pure mathematics , biology
A computer‐assisted method is presented for simultaneous optimization of pH and ion concentration selection for the optimal separation in reversed‐phase HPLC. The method is based on a polynomial estimation from nine preliminary experiments according to two‐factor rectangular design. This is followed by a two‐dimension computer scanning technique. Resolution is used as the selection criterion. Good agreement was obtained between predicted data and experimental results.