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The principle of optimization of binary mobile phase composition of multi‐step linear gradient elution
Author(s) -
Zou HanFa,
Zhang YuKui,
Dong LiFu,
Bao MianSheng,
Lu PeiZhang
Publication year - 1989
Publication title -
acta chimica sinica english edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 0256-7660
DOI - 10.1002/cjoc.19890070606
Subject(s) - chemistry , binary number , resolution (logic) , gradient elution , phase (matter) , chromatography , elution , column (typography) , composition (language) , high performance liquid chromatography , position (finance) , analytical chemistry (journal) , computer science , artificial intelligence , mathematics , connection (principal bundle) , linguistics , philosophy , arithmetic , organic chemistry , finance , economics , geometry
The basic principle of optimal method called “moving overlapping resolution mapping method” to select the optimal binary mobile phase composition of multi‐step linear gradient liquid chromatography is discussed with simultaneously considering effects of position of solute inside the column and mobile phase composition on peak resolution and retention value, then a BASIC program based on this principle is developed in IBM‐PC computer. The validities of both principle of optimization and BASIC program are confirmed by separation of samples containing bile acids and PAHs in RP‐HPLC.