Premium
Chemistry of trichosanthin: VII. The chemical cleavage of the tryptophanyl peptide bond in trichosanthin
Author(s) -
GuangXiang Luo,
LiQing Zhang,
ZiWei Gu,
RuiQing Qian,
Yu Wang
Publication year - 1988
Publication title -
acta chimica sinica english edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 0256-7660
DOI - 10.1002/cjoc.19880060414
Subject(s) - trichosanthin , chemistry , peptide , tryptophan , cleavage (geology) , peptide bond , residue (chemistry) , stereochemistry , peptide fragment , organic chemistry , amino acid , biochemistry , geotechnical engineering , fracture (geology) , engineering
The single tryptophan residue of trichosanthin was cleaved by the use of BNPS‐skatole reaction with STIG2 peptide fregment which was obtained from the tryptic digestion of N ‐succinyl trichosanthin. After separation, the N ‐terminal sequence of tryptophanyl C ‐side peptide was determined by use of manual DABITC‐PITC double coupling sequencing method as follows: ‐Leu‐Ala‐Leu‐Ser‐Lys‐Gln‐Ile‐Gln‐Ile‐Ala‐.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom