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Synthesis of nonanucleotide (14‐20) of the dihydrouridine (D) loop of yeast alanine transfer RNA
Author(s) -
DeHua Chen,
JuXing Lou,
BingHui Yang,
LianFen Wu,
FaXian Chen,
DeFu Liu
Publication year - 1985
Publication title -
acta chimica sinica english edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 0256-7660
DOI - 10.1002/cjoc.19850030112
Subject(s) - chemistry , rna ligase , phosphorylation , rna , ligation , biochemistry , alanine , transfer rna , polynucleotide , phosphatase , stereochemistry , microbiology and biotechnology , amino acid , biology , gene
This paper deals with the synthesis of nonanucleotide AGDCGGDAG p of the dihydrouridine (D) loop of yeast alanine transfer RNA by ligation of chemically or enzymatically synthesized AGDCGG with excessive *pDAG p in the presence of T 4 ‐RNA ligase and the choice of the optimum conditions of 5′‐phosphorylation and ligation of the fragments AG, AGDC, GG, GG p and DAG which were the constituents of the named nonanucleotide. The final product, nonanucleoside octaphosphate, was obtained by removing 3′‐terminal phosphate group of the nonanucleotide with polynucleotide kinase possessing 3′‐phosphatase activity and was checked by the sequence analysis in accordance with AGDCGGDAG. It was found that the yield of 5′‐phosphorylation of DMP or DAG were almost quantitative at 5‐10°C. The synthetic AGDCGGDAG p had been used for the total synthesis of t ‐RNA y A|a .