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Modelling Oxygen Transfer and Aerobic Growth in Shake Flasks and Well‐Mixed Bioreactors
Author(s) -
Nikakhtari Hossein,
Hill Gordon A.
Publication year - 2005
Publication title -
the canadian journal of chemical engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.404
H-Index - 67
eISSN - 1939-019X
pISSN - 0008-4034
DOI - 10.1002/cjce.5450830312
Subject(s) - laboratory flask , bioreactor , aeration , pseudomonas putida , chemistry , oxygen , chromatography , biochemistry , organic chemistry , enzyme
Oxygen transfer is an important aspect of aerobic metabolism. In this work, microbial growth on glucose (fast metabolism) and phenol (slow metabolism) have been studied using Pseudomonas putida in shake flasks and a mixed bioreactor considering both substrate and oxygen depletion. Under typical operating conditions, the highest mass transfer coefficient ( K L a ) for the aerated well‐mixed bioreactor was found to be 50.8 h −1 , while the maximum non‐aerated shake flask K L a was 21.1 h −1 . The presence of media and/or dead cells did not have significant effect on measured values of K L a . A new equation for prediction of K L a in shake flasks with an absolute average deviation of 11.1% is introduced, and a combined model for oxygen mass transfer and microbial growth is shown to fit experimental data during growth on glucose and phenol in both shake flasks and the mixed bioreactor with an absolute average deviation of 19.3%.