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Reverse Micellar Extraction of Lysozyme Using Two Dialkyl Sodium Phosphinates
Author(s) -
Shin YounOk,
Vera Juan H.
Publication year - 2004
Publication title -
the canadian journal of chemical engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.404
H-Index - 67
eISSN - 1939-019X
pISSN - 0008-4034
DOI - 10.1002/cjce.5450820216
Subject(s) - lysozyme , chemistry , chromatography , micellar solutions , aqueous solution , extraction (chemistry) , aqueous two phase system , micelle , sodium , micellar liquid chromatography , phase (matter) , organic chemistry , biochemistry
The extraction of lysozyme was studied using di‐(2,4,4‐trimethylpentyl) sodium phosphinate, R 2 POONa, and di‐(2,4,4‐trimethylpentyl) sodium dithiophosphinate, R 2 PSSNa. For a R 2 POONa reverse micellar system, all lysozyme was removed from the aqueous phase when the pH of the aqueous solution was set below the pI of the protein. The extraction of lysozyme into a R 2 PSSNa reverse micellar system was independent of the pH of the aqueous phase. A solubilization limit of lysozyme exists in the R 2 POONa reverse micellar phase. The lysozyme recovery was 70% from the R 2 POONa reverse micellar phase, and less than 10% from the R 2 PSSNa reverse micellar phase.