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Lag phase model for transient growth of pseudomonas putida on phenol
Author(s) -
Tarighian Alireza,
Hill Gordon,
Lin YenHan
Publication year - 2001
Publication title -
the canadian journal of chemical engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.404
H-Index - 67
eISSN - 1939-019X
pISSN - 0008-4034
DOI - 10.1002/cjce.5450790505
Subject(s) - pseudomonas putida , transient (computer programming) , lag , bioreactor , biological system , steady state (chemistry) , phenol , phase (matter) , substrate (aquarium) , work (physics) , continuous modelling , computer science , thermodynamics , chemistry , mathematics , physics , biology , ecology , computer network , biochemistry , mathematical analysis , organic chemistry , enzyme , operating system
The successful design of large‐scale bioreactors requires the ability to predict both steady‐state and dynamic operating conditions. At the same time, mathematical models should not be too complex in order to reduce experimental work required to determine kinetic parameters. A simple model which predicts the behaviour of batch and transient continuous culture operations is presented and experimentally verified. The model is based on two regions of metabolic activity: the lag phase and the active phase. Pseudomonas putida growing on phenol as a substrate in a well‐mixed bioreactor was tested in three modes of operation: batch, continuous start‐up and continuous step‐change. The model is demonstrated to predict all the qualitative aspects of the dynamic phases of growth and is quantitatively accurate.