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Plasmid stability analysis in a non‐homogeneous bioreactor for a recombinant fed‐batch fermentation
Author(s) -
Patnaik Pratap R.
Publication year - 1999
Publication title -
the canadian journal of chemical engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.404
H-Index - 67
eISSN - 1939-019X
pISSN - 0008-4034
DOI - 10.1002/cjce.5450770325
Subject(s) - bioreactor , dilution , fermentation , chemistry , recombinant dna , escherichia coli , zymomonas mobilis , chromatography , biology , biochemistry , organic chemistry , ethanol fuel , physics , gene , thermodynamics
Fed‐batch fermentation for tryptophan synthetase by a recombinant Escherichia coli strain has been analyzed through a model incorporating segregational loss of the plasmid pPLc23 trp Al and imperfect macromixing of the broth. These features become significant in large fermentation vessels, where fluid circulation in the bioreactor influences the rates of cell growth and productivity. A simple model consisting of two interacting reactors describes the degree of macromixing, which is characterized by the respective (internal) dilution rates. Controlled imperfect macromixing is superior to other methods in arresting, and even reversing, the normal decline in the concentration of recombinant cells, thereby providing a method to exploit the non‐homogeneity of the broth in large bioreactors. Some physical implications are discussed.