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Fabrication of amperometric biosensors by entrapment of enzymes in functionalized polypyrrole films
Author(s) -
Cosnier Serge
Publication year - 1998
Publication title -
the canadian journal of chemical engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.404
H-Index - 67
eISSN - 1939-019X
pISSN - 0008-4034
DOI - 10.1002/cjce.5450760605
Subject(s) - polypyrrole , biosensor , amperometry , immobilized enzyme , adsorption , monomer , electrode , electrochemistry , pyrrole , materials science , selectivity , amphiphile , conductive polymer , chemistry , fabrication , nanotechnology , combinatorial chemistry , chemical engineering , chromatography , polymer , organic chemistry , enzyme , copolymer , catalysis , alternative medicine , pathology , engineering , medicine
Abstract The concept and potentialities of an original electrochemical procedure of enzyme immobilization are described. This two‐step method consists of the adsorption of an aqueous amphiphilic pyrrole monomer‐enzyme mixture on an electrode surface followed by the electropolymerization of the adsorbed monomers. This procedure is compared with the conventional electrochemical methods of biosensor construction. Several bienzyme electrodes devoted to the improvement of the biosensor selectivity or to the amplification of the biosensor sensitivity are described. In addition, examples of organic phase enzyme electrode and the electrical wiring of immobilized enzymes are presented.