Premium
Stresses on immobilized phanerochaete chrysosporium hyphae in submerged cultures for ligninase production
Author(s) -
Zhen Zhonming,
Yu Jian
Publication year - 1998
Publication title -
the canadian journal of chemical engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.404
H-Index - 67
eISSN - 1939-019X
pISSN - 0008-4034
DOI - 10.1002/cjce.5450760414
Subject(s) - phanerochaete , chrysosporium , mycelium , aeration , hypha , protease , chemistry , shear stress , bioreactor , oxygen , microbiology and biotechnology , chromatography , food science , botany , enzyme , biology , materials science , biochemistry , composite material , organic chemistry
Effects of hydraulic shear stress and oxygen starvation stress on immobilized white‐rot fungus Phanerochaete chrysosporium in submerged cultures for ligninase production were studied. The immobilized fungal hyphae formed an even mycelial mat on a rotating cylindrical stainless steel net showing little sensitivity to the shear stress. The average activity of ligninase over 24 h during a peak production period was 389 U/L at a tip velocity of 0.44 m/s and 369 U/L at a doubled tip velocity of 0.88 m/s. When the dissolved oxygen level dropped below 1 mg/L, the fungal cells responded to the oxygen starvation stress and released a considerable amount of protease. The proteolytic denaturation of ligninase was the major reason that a negligible ligninase activity was detected at a low aeration rate.