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Effect of H 2 O 2 addition mode on enzymatic removal of phenol from wastewater in the presence of polyethylene glycol
Author(s) -
Wu Jiangning,
Bewtra J. K.,
Biswas N.,
Taylor K. E.
Publication year - 1994
Publication title -
the canadian journal of chemical engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.404
H-Index - 67
eISSN - 1939-019X
pISSN - 0008-4034
DOI - 10.1002/cjce.5450720515
Subject(s) - phenol , horseradish peroxidase , chemistry , polyethylene glycol , nuclear chemistry , stoichiometry , chromatography , peroxidase , enzyme , organic chemistry
The effect of batch and semi‐batch additions of the stoichiometric amount of H 2 O 2 on enzymatic removal of phenol has been studied over the phenol concentration range of 1‐10 mmol/L. It is found that the ratio between the maximum H 2 O 2 concentration during the reaction and the initial horseradish peroxidase concentration (( H 2 O 2 ) max /( HRP ) o ) controls the rate of phenol removal by horseradish peroxidase in the presence of the additive, polyethylene glycol. An optimum range of this ratio is determined to be between 10 and 25 μmol/U. Empirical models are proposed to predict the values of (H 2 O 2 ) max /(HRP) o required to treat given phenol concentrations under different modes for adding H 2 O 2 .