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Enzyme mass transfer coefficient in aqueous two phase system using a packed extraction column
Author(s) -
Patil T. A.,
Jafarabad K. Rostami,
Sawant S. B.,
Joshi J. B.
Publication year - 1991
Publication title -
the canadian journal of chemical engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.404
H-Index - 67
eISSN - 1939-019X
pISSN - 0008-4034
DOI - 10.1002/cjce.5450690221
Subject(s) - packed bed , chromatography , extraction (chemistry) , aqueous solution , mass transfer , mass transfer coefficient , chemistry , column (typography) , phase (matter) , aqueous two phase system , organic chemistry , computer science , telecommunications , frame (networking)
Fractional dispersed phase hold‐up and dispersed side mass transfer coefficients for amyloglucosidase were measured in a 56 mm i.d. packed extraction column using a sodium sulphate‐polyethylene glycol 4000 system. Raschig rings (3 to 13.3 mm), Berl saddles (12 mm), Pall rings (12.6 mm), glass spheres (5.2 mm) and structured wire gauze were used as packings. The effect of packing size was investigated in the case of ceramic Raschig rings. The effect of phase composition of the aqueous phase system also was studied. Correlations have been developed for fractional dispersed phase hold‐up and volumetric mass transfer coefficient with packing voidage, dry surface area of packings, superficial dispersed phase velocity and the liquid phase physical properties.