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The effect of specific growth rate and death rate on monoclonal antibody production in hybridoma chemostat cultures
Author(s) -
Linardos Theodor I.,
Kalogerakis Nicolas,
Behie Leo A.,
Lamontagne Louis R.
Publication year - 1991
Publication title -
the canadian journal of chemical engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.404
H-Index - 67
eISSN - 1939-019X
pISSN - 0008-4034
DOI - 10.1002/cjce.5450690205
Subject(s) - chemostat , monoclonal antibody , biology , antibody , chemistry , immunology , genetics , bacteria
Experimental data collected from bench‐scale chemostat cultures of mouse‐mouse hybridoma cells have been used for the development of a kinetic model of monoclonal antibody production. The specific antibody productivity was found to be proportional to the specific death rate, indicating a stimulating effect of stress on the ability of the cells to produce antibody. The death rate was found to be an exponential function of the average cell age in the culture. Furthermore, a Generalized Maintenance Energy (GME) model is proposed, to take account of the effects of culture stress on the nutrient uptake rates. The proposed relationships agreed very well with other mouse‐mouse hybridoma chemostat culture data in the literature.