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Dynamic analysis of a fluidized bed reactor for sucrose inversion catalyzed by immobilized invertase
Author(s) -
Doǧu Timur,
Kaletunç Gönül,
çaǧlar Arif
Publication year - 1985
Publication title -
the canadian journal of chemical engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.404
H-Index - 67
eISSN - 1939-019X
pISSN - 0008-4034
DOI - 10.1002/cjce.5450630111
Subject(s) - invertase , fluidized bed , chromatography , polyacrylamide , materials science , immobilized enzyme , chemistry , particle size , chemical engineering , dispersion (optics) , catalysis , sucrose , analytical chemistry (journal) , polymer chemistry , organic chemistry , enzyme , engineering , physics , optics
Abstract Axial mixing characteristics and performance of a liquid fluidized bed reactor for the sucrose inversion reaction, which was catalyzed by immobilized invertase was studied. The invertase enzyme was immobilized in polyacrylamide gel by the bead polymerization technique. Well‐defined spherical gel particles of five different sizes (0.29–3.16 mm) were prepared. Efficiency of the immobilization technique, the optimum working conditions and the kinetic parameters were determined in a batch system. It is shown that as the particle size increases the rate of inversion first increases due to decrease of enzyme loss by leaching and then decreases because of diffusional limitations after a maixmum is reached. The performance of the fluidized bed reactor was investigated dynamically by introducing a step input of substrate at the inlet and analyzing the response curves. These experiments were performed at the optimum temperature (55°C) and using the optimum particle size (2.15 mm). The axial dispersion coefficient was found to increase from 0.45 to 1.26 cm 2 /s by changing the liquid velocity from 0.32 to 0.58 cm/s.