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The production of the antibiotic patulin in a three phase fluidized bed reactor I. Effect of medium composition
Author(s) -
Berk Dimitrios,
Behie Leo A.,
Jones Alan,
Lesser Barry H.,
Maurice Gaucher G.
Publication year - 1984
Publication title -
the canadian journal of chemical engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.404
H-Index - 67
eISSN - 1939-019X
pISSN - 0008-4034
DOI - 10.1002/cjce.5450620118
Subject(s) - patulin , fluidized bed , chromatography , chemistry , effluent , phase (matter) , food science , pulp and paper industry , organic chemistry , environmental science , environmental engineering , mycotoxin , engineering
The continuous and semi‐batch production of the antibiotic patulin by immobilized Penicillium urticae cells in a three phase fluidized bed reactor was investigated. A key factor for the success of the process was the limitation of the nitrogen source which increased the longevity of the biocatalyst while keeping cell growth at a low level. The continuous reactor was operated for 360 h during which time the effluent concentration of patulin was maintained at 0.25 mmol/L for 192 h. The total amount of patulin produced by the continuuous run was 35% greater than that obtained in a semibatch run. The pathway efficiency was 0.35 for 192 h indicating the biosynthetic pathway was operating at a high catalytic efficiency.