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Fractionnement des mélanges de protéines par électrophorèse convective
Author(s) -
Sanchez Victor,
Casademont Ernest,
Mahenc Jean
Publication year - 1977
Publication title -
the canadian journal of chemical engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.404
H-Index - 67
eISSN - 1939-019X
pISSN - 0008-4034
DOI - 10.1002/cjce.5450550111
Subject(s) - convection , fractionation , chemistry , chromatography , countercurrent exchange , stack (abstract data type) , thermodynamics , physics , computer science , programming language
A new apparatus is proposed which allows the fractionation of protein mixtures by convective electrophoresis. The cell is arranged as a stack of elementary units, each of which consists of two compartments bounded by semi‐permeable membranes and separated by a permeable cotton diaphragm. The solutions are circulated by forced convection, either co‐current or eounter‐current. The development of transport equations has made it possible to define which parameters control separation. An experimental study has been carried out to show the effect of these parameters (potential difference, rate of convection, number of elementary units) on the concentration of proteins from dilute solutions and on the fractionation of binary mixtures. Finally, predictive calculations are attempted in order to define the optimum operating conditions, and in order to scale‐up the apparatus used.