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The effects of intraparticle diffusion on the kinetics of gel entrapped enzymes
Author(s) -
Korus R.,
O'Driscoll K. F.
Publication year - 1974
Publication title -
the canadian journal of chemical engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.404
H-Index - 67
eISSN - 1939-019X
pISSN - 0008-4034
DOI - 10.1002/cjce.5450520611
Subject(s) - diffusion , chemistry , glucose oxidase , enzyme , kinetics , michaelis–menten kinetics , immobilized enzyme , trypsin , urease , enzyme kinetics , particle (ecology) , chemical engineering , chromatography , biochemistry , thermodynamics , enzyme assay , biology , active site , ecology , engineering , physics , quantum mechanics
Abstract The results of a theoretical treatment are given for the effect of intraparticle diffusion on the Michaelis‐Menten kinetic behaviour of enzymes entrapped in spherical hydrogel particles. It is shown that curvature in Lineweaver‐Burk plots may be expected, and that the influence of particle size on the activity of entrapped enzymes may be predicted. The effectiveness factor is discussed and analogies with heterogeneous catalysis noted. Experimental results are presented for the hydrolyses of model substrates by gel entrapped trypsin, β‐galactosidase, glucose oxidase and urease which are consistent with the theoretical treatment.