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Bio‐scouring of cotton fabric and enzymatic degumming of jute fibres by a thermo‐alkaline recombinant rhamnogalacturonan lyase, ctrglf from Clostridium thermocellum
Author(s) -
Dhillon Arun,
Rajulapati Vikky,
Goyal Arun
Publication year - 2019
Publication title -
the canadian journal of chemical engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.404
H-Index - 67
eISSN - 1939-019X
pISSN - 0008-4034
DOI - 10.1002/cjce.23342
Subject(s) - clostridium thermocellum , chemistry , enzyme , lyase , food science , horticulture , biochemistry , cellulase , biology
In this study, the use of recombinant rhamnogalacturonan lyase Ct RGL f in bio‐scouring of cotton and enzymatic degumming jute was evaluated. The best concentration for bio‐scouring of cotton and degumming of jute was found to be 12 mg/mL (3.2 U/mL) of crude Ct RGL f . Cotton fabric treated with 12 mg/mL (3.2 U/mL) of crude Ct RGL f took 30 s to absorb a drop of water while the Ct RGL f untreated fabric took 40 min. The crude Ct RGL f reduced the water absorption time of cotton fabric by 98 % after 45 min of treatment. FESEM images showed that crude Ct RGL f (12 mg/mL; 3.2 U/mL), effectively removed the waxy compounds from the surface of jute fibres after 1 h of treatment. These results showed that Ct RGL f is a potentially important enzyme for the textile industry.