Bio‐scouring of cotton fabric and enzymatic degumming of jute fibres by a thermo‐alkaline recombinant rhamnogalacturonan lyase, ctrglf from Clostridium thermocellum

In this study, the use of recombinant rhamnogalacturonan lyase Ct RGL f in bio‐scouring of cotton and enzymatic degumming jute was evaluated. The best concentration for bio‐scouring of cotton and degumming of jute was found to be 12 mg/mL (3.2 U/mL) of crude Ct RGL f . Cotton fabric treated with 12 mg/mL (3.2 U/mL) of crude Ct RGL f took 30 s to absorb a drop of water while the Ct RGL f untreated fabric took 40 min. The crude Ct RGL f reduced the water absorption time of cotton fabric by 98 % after 45 min of treatment. FESEM images showed that crude Ct RGL f (12 mg/mL; 3.2 U/mL), effectively removed the waxy compounds from the surface of jute fibres after 1 h of treatment. These results showed that Ct RGL f is a potentially important enzyme for the textile industry.