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Folate functionalized silicon nanowires with highly enhanced adhesion to cancer cells
Author(s) -
Shadmani Saeid,
Salehi Zeinab,
Doosthosseini Hamid,
Mohajerzadeh Shams,
Roozbahani Sahar
Publication year - 2018
Publication title -
the canadian journal of chemical engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.404
H-Index - 67
eISSN - 1939-019X
pISSN - 0008-4034
DOI - 10.1002/cjce.22926
Subject(s) - surface modification , silanization , triethoxysilane , materials science , fourier transform infrared spectroscopy , chemical engineering , nanotechnology , adhesion , transmission electron microscopy , attenuated total reflection , nanowire , composite material , engineering
In this study, we implemented a functionalization process on the surface of silicon nanowires (SiNWs) which were fabricated by chemical vapour deposition (CVD) method in a low‐pressure CVD (LPCVD) chamber. Surface functionalization was carried out in three stages: hydroxylation of the surface with piranha solution, silanization with a solution of 3‐Aminopropyl triethoxysilane (APTES) in n ‐hexane, and finally functionalization with folic acid as a detection agent. Optical spectroscopy and electron microscopy techniques were used to experimentally characterize materials. Surface functionalization was evaluated using Attenuated Total Reflectance Fourier‐Transform Infrared spectroscopy (FTIR‐ATR), Field Emission Scanning Electron Miscoscopy (FESEM), and Transmission Electron Microscopy (TEM). Cell adhesion to functionalized and non‐functionalized silicon nanowires was compared; a cell assay analysis on lung metastatic cells was conducted. Lung cancer metastatic QUDB cells were seeded on a SiNWs surface in RPMI1640 complete medium. To evaluate the adhesion of cancer cells to these surfaces FESEM imaging was used. The number of cancer cells trapped on the folic acid functionalized SiNWs was found to be approximately 3 times higher than that of the non‐functionalized ones.