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Immobilization of horseradish peroxidase on modified PAN‐based membranes for the removal of phenol from buffer solutions
Author(s) -
Wang Shuai,
Liu Wei,
Zheng Jinwang,
Xu Xiaoping
Publication year - 2016
Publication title -
the canadian journal of chemical engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.404
H-Index - 67
eISSN - 1939-019X
pISSN - 0008-4034
DOI - 10.1002/cjce.22469
Subject(s) - horseradish peroxidase , phenol , buffer (optical fiber) , membrane , chemistry , peroxidase , chromatography , chemical engineering , organic chemistry , computer science , biochemistry , enzyme , engineering , telecommunications
Polyacrylonitrile ultrafiltration membranes have been widely used in many separation processes. In this paper, horseradish peroxidase was immobilized onto polyacrylonitrile ultrafiltration membrane by crosslinking with glutaraldehyde. The immobilized enzyme possessed a protein loading of 0.025 mg/cm 2 membrane and a specific activity of 105 U/mg protein (105 μmol/min/mg protein ). The initial modified and immobilized membranes were observed using SEM and FTIR. Potential applications of HRP membranes were investigated in the removal of phenol through oxidation with the addition of hydrogen peroxide. The optimum pH of the immobilized enzyme was determined to be 6.0, and the optimum hydrogen peroxide concentration to be 30 mmol/L. Almost 100 % removal of phenol (1 ∼ 10 mg/L) from water was achieved by HRP membranes. For high concentrated solutions, successive cycles were successfully used to improve the degree of phenol oxidation. Furthermore, the immobilized horseradish peroxidase was operationally stable. These results suggest that the HRP membrane has promising applications for the removal of phenol.