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Cultivation of freshwater green alga Neochloris oleoabundans in non‐sterile media co‐inoculated with protozoa
Author(s) -
Peng Licheng,
Lan Christopher Q.,
Zhang Zisheng
Publication year - 2016
Publication title -
the canadian journal of chemical engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.404
H-Index - 67
eISSN - 1939-019X
pISSN - 0008-4034
DOI - 10.1002/cjce.22407
Subject(s) - protozoa , sodium bicarbonate , biology , inoculation , incubation , bicarbonate , algae , botany , microbiology and biotechnology , food science , chemistry , horticulture , biochemistry , endocrinology
The effects of sodium bicarbonate on freshwater green alga Neochloris oleoabundans were evaluated with respect to cell growth, cell morphology, and control of protozoa contamination under non‐sterile conditions co‐inoculated with protozoa pre‐cultures. While culture collapse occurred in the control and the cultures containing 40 mmol/L NaHCO 3 due to protozoa contamination, the addition of 160 or 200 mmol/L NaHCO 3 could completely inhibit the growth of microalgae‐preying protozoa and allow safe cultivation of N. oleoabundans for co‐incubation with protozoa in non‐sterile media. These results suggest that well‐formulated media containing appropriate amounts of dissolved inorganic carbon (DIC) may enable low‐cost cultivation of freshwater microalgae in non‐sterile open systems.