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Growth optimization of Synechococcus elongatus PCC7942 in lab flasks and a 2‐D photobioreactor
Author(s) -
Kuan David,
Duff Sheldon,
Posarac Dusko,
Bi Xiaotao
Publication year - 2015
Publication title -
the canadian journal of chemical engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.404
H-Index - 67
eISSN - 1939-019X
pISSN - 0008-4034
DOI - 10.1002/cjce.22154
Subject(s) - photobioreactor , light intensity , growth rate , laboratory flask , biomass (ecology) , chemistry , airlift , photosynthesis , cyanobacteria , volumetric flow rate , bacterial growth , botany , zoology , chromatography , bioreactor , biology , bacteria , ecology , physics , geometry , mathematics , genetics , optics , quantum mechanics
This study explored the effects of the major growth parameters for Synechococcus elongatus PCC7942 for the purpose of maximizing cell growth. Preliminary studies in shake flasks revealed that an optimization of components in BG‐11 growth medium resulted in no significant improvements for the specific growth rate and biomass concentration. However, an optimal temperature of 33 °C and light intensity of 120 μE/m 2 ·s provided a maximum specific growth rate of 0.052 h ‐1 and a maximum biomass concentration of 0.50 g/L. Further studies of light intensity, CO 2 concentration, and gas flow rate in a 1 L airlift photobioreactor showed that air enriched with 5 % CO 2 with a flow rate of 1 L/min, temperature of 33 °C, and light intensity of 6 μE/m 2 ·s achieved a maximum in biomass concentration of 1.07 g/L with a specific growth rate of 0.026 h ‐1 . The specific growth rate and biomass concentration diminished when CO 2 concentration and light intensity increased above 5 % and 120 μE/m 2 ·s, respectively. CO 2 absorption experiments also revealed that growth was significantly limited by CO 2 when supplied with air, whereas 10 % CO 2 lowered the pH and consequently reduced the specific growth rate. Application of a 12:12 hour light‐dark cycle resulted in a significant drop in biomass growth.