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Induction and suppression of Dichomitus squalens and Ceriporiopsis subvermispora peroxidase activity by manganese sulphate in response to carbon and nitrogen sources
Author(s) -
Kannaiyan Ranjani,
Mahinpey Nader,
Martinuzzi Robert J.,
Kostenko Victoria
Publication year - 2014
Publication title -
the canadian journal of chemical engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.404
H-Index - 67
eISSN - 1939-019X
pISSN - 0008-4034
DOI - 10.1002/cjce.21935
Subject(s) - peroxidase , manganese peroxidase , chemistry , lignocellulosic biomass , nitrogen , biomass (ecology) , enzyme , food science , manganese , biochemistry , environmental chemistry , agronomy , fermentation , biology , organic chemistry
Delignification is critical in the production of biofuel from a potentially abundant renewable resource, lignocellulosic waste. One of the classes of lignolytic enzymes are peroxidases. Manganese sulphate (Mn 2+ ) has a significant impact on the peroxidase activity of lignolytic fungi. Along with induction, the suppression of peroxidase activity by Mn 2+ has been observed. Peroxidase regulation is governed by three critical Mn 2+ concentrations: the minimum inductive concentration (MIC), the peak concentration (PC) and the minimum suppressive concentration (MSC). The induction and suppression of enzyme activity were not associated with fungal growth capacity, but with a specific enzyme response to the nutritional conditions. Manipulation of the carbon and nitrogen sources shifted the peroxidase suppression by Mn 2+ to high concentrations and, hence, increased the peroxidase tolerance to Mn 2+ and, consequently, peak peroxidase activities. The manipulation of carbon and nitrogen sources allowed increasing the peak concentrations of Mn 2+ and corresponding peroxidase activity up to 0.5 mg/mL and 180.70 AU (173% increase compared to standard) in C. subvermispora , and up to 0.05 mg/mL and 151.23 AU in D. squalens (91% increase compared to standard). Thus, manipulation of carbon and nitrogen sources is a useful tool in enhancing fungi peroxidase tolerance to Mn 2+ and improving the delignification of lignocellulosic biomass by fungi to prepare an easily consumable substrate for economically viable biofuel production.