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CO 2 sequestration using principles of shell formation
Author(s) -
Lee SeungWoo,
Lee SiHyun,
Jang YoungNam,
Lim KyoungSoo,
Jeong SoonKwan
Publication year - 2011
Publication title -
the canadian journal of chemical engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.404
H-Index - 67
eISSN - 1939-019X
pISSN - 0008-4034
DOI - 10.1002/cjce.20432
Subject(s) - chemistry , carbonic anhydrase , protein secondary structure , carbon dioxide , circular dichroism , carbonic acid , calcium carbonate , biochemistry , enzyme , organic chemistry
Biomimetic sequestration of carbon dioxide (CO 2 ) is one of the methods proposed to reduce the CO 2 released into the atmosphere. In this study, we compared soluble protein of hemocyte from diseased shell (HDS) and extrapallial fluid (EPF) extracted from Crassostrea gigas with bovine carbonic anhydrase II in terms of their ability to promote CO 2 hydration and the production of calcium precipitates. On the basis of the experiments of CO 2 hydration, the key role of HDS was identified. Moreover, mass‐spectroscopic analysis (MALDI‐TOF) and circular dichroism (CD) analysis were used for understanding molecular weight and secondary protein structure. From the amino acid sequence and secondary protein structure, the different processes of CO 2 hydration by bovine carbonic anhydrase II and HDS could be assessed.