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Influence of medium composition on oxygen transfer rate in animal cell culture
Author(s) -
Toye D.,
Galifi A.,
Salmon T.,
Marchot P.,
Verdin E.,
Crine M.
Publication year - 2010
Publication title -
the canadian journal of chemical engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.404
H-Index - 67
eISSN - 1939-019X
pISSN - 0008-4034
DOI - 10.1002/cjce.20302
Subject(s) - aeration , bubble , coalescence (physics) , mass transfer , oxygen , mass transfer coefficient , composition (language) , surface tension , viscosity , oxygen tension , chemistry , chemical engineering , aqueous solution , chromatography , thermodynamics , materials science , analytical chemistry (journal) , mechanics , organic chemistry , composite material , biology , linguistics , physics , philosophy , astrobiology , engineering
Experiments were conducted in a 0.25 m diameter bubble column to investigate the effect of medium composition on oxygen transfer rate. Aqueous solutions, the composition of which mimics a mammalian cell culture medium, are used. The effect on oxygen transfer rate of additives used to protect cells against local hydrodynamic stresses induced by bubble coalescence and bursting is addressed, in the range of operating conditions (aeration rates) met in animal cell cultures. The mass transfer coefficient, the liquid viscosity and surface tension, and the bubble size distribution are measured as a function of liquid composition and of gas superficial velocity, allowing to decouple the effects of the different additive on k L and on a .