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Impact of Bioreactor Geometry on Mesenchymal Stem Cell Production in Stirred‐Tank Bioreactors
Author(s) -
Petry Florian,
Salzig Denise
Publication year - 2021
Publication title -
chemie ingenieur technik
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.365
H-Index - 36
eISSN - 1522-2640
pISSN - 0009-286X
DOI - 10.1002/cite.202100041
Subject(s) - microcarrier , bioreactor , mesenchymal stem cell , process (computing) , process engineering , biochemical engineering , materials science , chemistry , computer science , engineering , cell , microbiology and biotechnology , biology , biochemistry , organic chemistry , operating system
Human mesenchymal stem cells (hMSCs) are manufactured as cell therapy products. This requires a tightly controlled and fully understood production process, usually taking place in a stirred‐tank bioreactor. In this review, the impact of bioreactor geometry as well as equipment‐related parameters is discussed. We provide a theoretical background for the engineering of hMSC spheroids with a defined size and evaluate the process parameters needed for the microcarrier‐based expansion of hMSCs. Finally, and based on experimental data, ideal bioreactor setups for each type of process are recommended.