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Study of Protein Adsorption During Sterile Filtration of Protein Formulations by ILC
Author(s) -
Haindl Susanne Maria Hilda,
Doppleb Olivia,
Förster Lucas,
Wraage Sophia,
Reiche Annette
Publication year - 2020
Publication title -
chemie ingenieur technik
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.365
H-Index - 36
eISSN - 1522-2640
pISSN - 0009-286X
DOI - 10.1002/cite.201900185
Subject(s) - adsorption , chromatography , filtration (mathematics) , size exclusion chromatography , chemistry , fouling , bovine serum albumin , protein adsorption , membrane fouling , membrane , elution , biochemistry , organic chemistry , statistics , mathematics , enzyme
Protein adsorption is usually regarded as the main reason for filter fouling in sterile filtration of protein formulations. To achieve a better insight into this phenomenon, protein adsorption was studied during filtration of stabilized bovine serum albumin (BSA) and γ ‐globulin formulations through 0.2‐µm microfilter membranes by inverse liquid chromatography (ILC). Adsorption processes can be studied with this method by measurement of breakthrough curves. The change of the concentration in the fluid phase is measured with high accuracy by an inline UV‐detector.