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Novel In Situ Magnetic Separator for Magnetic Particle‐Based Enzyme Purification or Biocatalysis
Author(s) -
Shaikh Yonas S.,
Seibert Christian,
Kampeis Percy
Publication year - 2018
Publication title -
chemie ingenieur technik
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.365
H-Index - 36
eISSN - 1522-2640
pISSN - 0009-286X
DOI - 10.1002/cite.201600107
Subject(s) - separator (oil production) , bioreactor , magnetic nanoparticles , magnetic separation , chromatography , chemistry , biocatalysis , materials science , particle (ecology) , in situ , magnetic particle inspection , chemical engineering , nanotechnology , nanoparticle , thermodynamics , catalysis , physics , engineering , organic chemistry , reaction mechanism , oceanography , geology , metallurgy
A novel in situ magnetic separator is presented in which the separation unit is directly mounted on the bioreactor. Therefore, it is possible to collect the magnetic particles directly from the reaction liquid and transfer them in one step sterile out of the reactor. By consecutive flushing, the particles are concentrated and separated from the rest. The operating parameters were determined and evaluated experimentally and by computational fluid dynamics. The separator was tested in combination with a 5‐L bioreactor with glass flask. Hereby, the accumulation kinetics of the particles were analyzed and used for a simple theoretical model.