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Development of the Travelling Wave Bioreactor. Part II: Engineering Characteristics and Cultivation Results
Author(s) -
Kaiser Stephan C.,
Perepelitsa Nadezda,
Kraume Matthias,
Eibl Dieter
Publication year - 2016
Publication title -
chemie ingenieur technik
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.365
H-Index - 36
eISSN - 1522-2640
pISSN - 0009-286X
DOI - 10.1002/cite.201500091
Subject(s) - bioreactor , traveling wave , suspension (topology) , suspension culture , mixing (physics) , mass transfer , substrate (aquarium) , biochemical engineering , process engineering , engineering , chemistry , chromatography , cell culture , biology , mathematics , physics , botany , ecology , mathematical analysis , genetics , quantum mechanics , homotopy , pure mathematics
The innovative travelling wave bioreactor (TWB), an orbitally shaken system, developed specifically for cell culture applications, is investigated. In this study, engineering parameters covering power input, mixing time and oxygen mass transfer obtained in two TWB prototypes are presented for the first time. Based on the results, cultivation parameters for CHO suspension cell cultivations were defined. Cell growth and substrate consumption were comparable with a wave‐mixed reference bioreactor, which demonstrates the bioreactor's applicability for cultivations of mammalian cell cultures for the first time.