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Ion‐Exchange Adsorption of Proteins: Experiments and Molecular Dynamics Simulations
Author(s) -
Liang Juan,
Fieg Georg,
Jakobtorweihen Sven
Publication year - 2015
Publication title -
chemie ingenieur technik
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.365
H-Index - 36
eISSN - 1522-2640
pISSN - 0009-286X
DOI - 10.1002/cite.201400095
Subject(s) - adsorption , steric effects , molecular dynamics , chemistry , thermodynamics , chromatography , albumin , ion , ion exchange , computational chemistry , organic chemistry , physics , biochemistry
The adsorption of serum albumin and hemoglobin onto Q Sepharose FF was investigated, where the single and binary adsorption were considered. Both experiments and molecular dynamics simulations have been performed to study the adsorption from macroscopic and microscopic points of view. The steric mass‐action model was applied to describe the single adsorption isotherms and to predict the binary isotherms. The results of the experiments and simulations were connected and compared by two parameters of the steric mass‐action model. Both results present a preferential adsorption of serum albumin.