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Fluid Flow and Cell Proliferation of Mesenchymal Adipose‐Derived Stem Cells in Small‐Scale, Stirred, Single‐Use Bioreactors
Author(s) -
Kaiser Stephan,
Jossen Valentin,
Schirmaier Carmen,
Eibl Dieter,
Brill Silke,
van den Bos Christian,
Eibl Regine
Publication year - 2013
Publication title -
chemie ingenieur technik
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.365
H-Index - 36
eISSN - 1522-2640
pISSN - 0009-286X
DOI - 10.1002/cite.201200180
Subject(s) - microcarrier , impeller , bioreactor , suspension (topology) , turbulence , fluid dynamics , shear stress , computational fluid dynamics , materials science , particle image velocimetry , mesenchymal stem cell , mechanics , chemistry , cell , physics , biology , microbiology and biotechnology , biochemistry , mathematics , composite material , organic chemistry , homotopy , pure mathematics
The fluid flow and suspension characteristics inside small‐scale, stirred, single‐use bioreactors were investigated experimentally and by means of computational fluid dynamics. The required impeller speeds for homogenous suspension were determined for two microcarrier types. The shear stress level and turbulence distribution were predicted using a numerical model, which was verified by particle image velocimetry measurements. In subsequent cultivations of primary mesencymal adipose‐derived stem cells, up to 31.4‐fold expansion in cell number was achieved for serum concentrations as low as 5 %.