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Wave‐Mixed and Orbitally Shaken Single‐Use Photobioreactors for Diatom Algae Propagation
Author(s) -
Lehmann Nicolai,
Rischer Heiko,
Eibl Dieter,
Eibl Regine
Publication year - 2013
Publication title -
chemie ingenieur technik
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.365
H-Index - 36
eISSN - 1522-2640
pISSN - 0009-286X
DOI - 10.1002/cite.201200137
Subject(s) - photobioreactor , airlift , phaeodactylum tricornutum , bioreactor , aeration , biomass (ecology) , diatom , algae , chromatography , botany , materials science , pulp and paper industry , chemistry , environmental science , biology , ecology , engineering
Although vertical column, flat‐plate, tubular, bubble column and airlift photobioreactors are widely used for the cultivation of diatoms, it has been shown that bubble‐induced hydrodynamic stress created in these types of bioreactor can damage cells. Therefore, three single‐use surface‐aerated bag bioreactors, known for their outstanding results in cultivating shear‐sensitive mammalian cells, were for the first time investigated for their suitability in growing the model microalgae Phaeodactylum tricornutum . All of the systems, which were additionally aerated with CO 2 and equipped with illumination systems providing different light qualities, guaranteed a 22‐ to 43‐fold increase in cell density within seven days, without any addition of cell protection agents or changes in cell morphology. Maximum cell density and dry biomass were achieved in the orbitally shaken 2D‐bag by using cool‐white fluorescent tubes.