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Increase of Protein Yield in High Five Cells in a Single‐Use Perfusion Bioreactor by Medium Replacement
Author(s) -
Rausch Mareike,
Pörtner Ralf,
Knäblein Jörg
Publication year - 2013
Publication title -
chemie ingenieur technik
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.365
H-Index - 36
eISSN - 1522-2640
pISSN - 0009-286X
DOI - 10.1002/cite.201200121
Subject(s) - bioreactor , heterologous , scalability , process engineering , biochemical engineering , yield (engineering) , process (computing) , computer science , protein expression , single use , scale up , microbiology and biotechnology , biomedical engineering , chemistry , engineering , materials science , biology , biochemistry , physics , organic chemistry , classical mechanics , database , metallurgy , gene , operating system
Abstract A multitude of heterologous proteins is needed in pharmaceutical research. These have to be produced in robust and flexible cultivation systems to allow a fast and cost‐effective handling. Moreover, these systems should be scalable and meet quality and regulatory requirements. The baculovirus expression vector system propagated on High Five insect cells is a popular choice for this purpose. For this expression system an efficient, robust and scalable process was developed based on a single‐use technology and a medium replacement strategy with cell retention. It is shown that a medium replacement in the Wave bioreactor is feasible and can be scaled up to a 10‐L scale without much effort.