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Micellar electrokinetic capillary chromatography determination of (+)S and (−)R arotinolol in serum using UV detection and solid phase extraction
Author(s) -
Hefnawy Mohammed M.
Publication year - 2001
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.888
Subject(s) - chemistry , chromatography , detection limit , micellar electrokinetic chromatography , capillary electrophoresis , extraction (chemistry) , analytical chemistry (journal) , enantiomer , solid phase extraction , resolution (logic) , calibration curve , analyte , electrokinetic phenomena , stereochemistry , artificial intelligence , computer science
A method for the simultaneous determination of (+)S and (−)R arotinolol in serum by micellar electrokinetic capillary chromatography is described. Stereoselective resolution of the arotinolol enantiomers was achieved using 5 mM sodium taurocholate in 10 mM sodium dihydrogen phosphate buffer of pH 2.5. A 72‐cm uncoated fused‐silica capillary at a constant voltage of 15 kV was used for the analysis. The analytes of interest were extracted from serum using solid phase extraction. An octadecyl cartridge gave good recoveries in excess of 87% for both (+)S and (−)R arotinolol without any interference. The calibration curves were linear over the range of 50–500 ng ml −1 with (+)S propranolol as the internal standard and the coefficient of determination was greater than 0.999 (n = 3). The limit of quantitation was 50 ng ml −1 for each enantiomer and the detection limit using 1 ml serum and a UV detection set et 220 nm was 25 ng ml −1 (S/N = 2). Precision and accuracy of the method were in the range 0.8–2.7% and 1.2–6.4%, respectively, for (+)S arotinolol and 1.1–3.9% and 2.2–6.5%, respectively, for (−)R arotinolol. Chirality 14:67–71, 2002. © 2002 Wiley‐Liss, Inc.