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In vitro fenoprofenyl–coenzyme a thioester formation: Interspecies variations
Author(s) -
Soraci A.,
Benoit E.
Publication year - 1995
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.530070707
Subject(s) - chemistry , palmitic acid , thioester , microsome , substrate (aquarium) , isozyme , coenzyme a , in vitro , stereochemistry , biochemistry , guinea pig , enzyme , fatty acid , reductase , endocrinology , biology , ecology
In vitro coenzyme A thioester formation from (−)‐(R)‐fenoprofen (FPF) and palmitic acid has been studied using liver microsomes from rat, guinea pig, sheep, and dog. In every species with both palmitic acid or (−)‐(R)‐fenoprofen, the Lineweaver–Burk plot was linear in the substrate concentration range used and as a consequence agrees with the involvement of only one isoenzyme (or different isoenzymes of similar K m values). The V max values for the thioesterification of (−)‐(R)‐fenoprofen present large species variations from 2.1 ± 1.0 with sheep liver microsomes to 60.6 ± 11 nmol/min/mg with dog liver microsomes. These values statistically significantly correlate ( r = 0.94) to the V max values observed when palmitic acid was used as a substrate. Furthermore palmitic acid inhibited (−)‐(R)‐fenoprofen–CoA formation in the same extent in all animal species. The stereoselectivity of the thioesterification was also species dependent. © 1995 Wiley‐Liss, Inc.