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Determination of the enantiomeric purity of dihydroxy‐ and dimethoxy‐2‐aminotetralins by high‐performance capillary electrophoresis with cyclodextrins as chiral selector
Author(s) -
Castelnovo Pierfrancesco,
Albanesi Carlo
Publication year - 1995
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.530070612
Subject(s) - chemistry , capillary electrophoresis , enantiomer , chromatography , cyclodextrin , resolution (logic) , detection limit , selectivity , bromide , organic chemistry , artificial intelligence , computer science , catalysis
A chiral separation of 5,6‐ and 6,7‐dihydroxy‐2‐aminotetralin and of the corresponding dimethoxy analogues can be achieved by high‐performance capillary electrophoresis using cyclodextrins as chiral selector. The selectivity of a number of native and derivatized cyclodextrins was screened and hydroxylalkyl‐β‐cyclodextrins turned out to be the most effective. Optimization of hydroxyethylcyclodextrin and hydroxypropylcyclodextrin concentration for each compound led to baseline separation (resolution factors from 1.7 to 2.3) resulting in a detection limit of 0.1% for the enantiomer present as impurity. Modifiers of the electroendosmotic flow, such as hydroxypropylcellulose and hexadecyltrimethylammoniun bromide, must be added to the background electrolyte to obtain such results. © 1995 Wiley‐Liss, Inc.