Premium
A comparison of LC and SFC for cellulose‐ and amylose‐derived chiral stationary phases
Author(s) -
BargmannLeyder Nathalie,
Tambuté André,
Caude Marcel
Publication year - 1995
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.530070502
Subject(s) - chemistry , supercritical fluid chromatography , enantiomer , cellulose , chromatography , amine gas treating , chiral column chromatography , polar , chirality (physics) , stereoselectivity , resolution (logic) , organic chemistry , selectivity , high performance liquid chromatography , chiral symmetry , physics , quantum mechanics , astronomy , artificial intelligence , computer science , nambu–jona lasinio model , quark , catalysis
This paper presents a systematic comparison of liquid chromatography (LC) and supercritical fluid chromatography (SFC) for Chiralcel OD and Chiralpak AD chiral stationary phases (CSPs), performed using various chiral compounds having a known or potential pharmaceutical activity. The chiral recognition mechanisms involved in LC and SFC for the enantiomeric separation of β‐blockers have been studied more particularly. As a general rule, it appears that the presence of polar functions, like primary or secondary hydroxyl or amine functions, may result in marked discrepancies in selectivity between LC and SFC. This result is peculiar to cellulose‐ and amylose‐derived CSPs, for which the interactions involved in chiral recognition mechanism are not always well balanced, contrary to what happens for independent CSPs. In the case of chiral resolution of polar solutes or polymer‐type CSPs, the analyst should try both the LC and SFC techniques to be able to choose the more stereoselective one. © 1995 Wiley‐Liss, Inc.