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Enantioselectivity suggests a cytosolic origin for a commercial pig liver esterase preparation
Author(s) -
Yang Shen K.,
Chen Shuju,
Huang Jinding
Publication year - 1995
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.530070108
Subject(s) - esterase , chemistry , pig liver , cytosol , microsome , hydrolysis , chromatography , guinea pig , enantiomer , biochemistry , enzyme , specific activity , oxazepam , stereochemistry , endocrinology , biology , receptor , benzodiazepine
A widely utilized pig liver esterase preparation has been found to be derived essentially exclusively from the cytosolic fraction of pig livers. Esterases in cytosol and microsomes prepared from a fresh pig liver hydrolyzed the S ‐ and R ‐enantiomers of racemic oxazepam 3‐acetate ( rac ‐OXA) with specific activity ratios of approximately 2.3:1 and 1:62, respectively. Product formations were analyzed by chiral stationary phase high‐performance liquid chromatography. The commercial pig liver esterase preparation showed greater activity toward S ‐OXA than did the esterases in the cytosolic fraction prepared from fresh pig liver. The results established that (i) esterases contained in microsomes and cytosol of pig liver have opposite enantioselectivity in the hydrolysis of rac ‐OXA and (ii) the commercial pig liver esterase preparation has a cytosolic origin. © 1995 Wiley‐Liss, Inc.