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Direct resolution of ergot alkaloid enantiomers on a novel chiral silica‐based stationary phase
Author(s) -
Flieger Miroslav,
Sinibaldi Massimo,
Cvak Ladislav,
Castellani Loredana
Publication year - 1994
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.530060707
Subject(s) - chemistry , enantiomer , resolution (logic) , silica gel , alkaloid , chiral stationary phase , chiral column chromatography , high performance liquid chromatography , chiral resolution , methanol , derivative (finance) , chromatography , lisuride , phase (matter) , organic chemistry , biochemistry , bromocriptine , artificial intelligence , computer science , prolactin , financial economics , hormone , economics
A new covalently‐bonded, silica‐based stationary phase, using as the chiral selector the 1‐(3‐aminopropyl) derivative of (+)‐(5R,8S,10R)‐terguride, has been developed to resolve optically active isomers by HPLC. Good resolution of structurally related racemic ergot alkaloids were obtained using water‐methanol mixtures as the eluent. Analysis of the influence of the type and concentration of the organic modifier, and the pH of the buffer in the mobile phase allowed the enantioseparation of these compounds to be optimized. Determination of the optical purity of a lisuride‐containig drug is reported. © 1994 Wiley‐Liss, Inc.