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Preparation of milligrams of pure enantiomers using analytical‐scale high‐performance liquid chromatography
Author(s) -
Liu Gaoyuan,
Goodall David M.,
Hunter Anna T.,
Massey Peter R.
Publication year - 1994
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.530060412
Subject(s) - chemistry , enantiomer , chromatography , resolution (logic) , high performance liquid chromatography , analytical chemistry (journal) , chiral column chromatography , organic chemistry , artificial intelligence , computer science
Pure enantiomers of an agrochemical process intermediate, (RS)‐1‐(4‐chlorophenyl)‐4,4‐dimethyl‐2‐(1 H ‐1,2,4‐triazol‐1‐yl)‐pentan‐3‐one ( 1 ), have been prepared on the milligram scale under overload chromatographic conditions on an analytical chiral column (250 × 4.6 mm i.d.). The effects of variation of temperature and mobile phase composition on retention factor, separation factor, and peak resolution have been investigated. Effects of flow rate, enantiomer ratio, sample concentration, and column load on productivity are also studied. Seven milligrams of the less retained (+)‐enantiomer and 5 mg of the (−)‐enantiomer were obtained from a single injection of 21 mg of (RS)‐ 1 . © 1994 Wiley‐Liss, Inc.