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Enantiomeric LC separation of calcium antagonists on protein‐based chiral stationary phases
Author(s) -
De Lorenzi Ersilia,
Fell Anthony F.,
Holmes Alan L.,
Caccialanza Gabriele,
Massolini Gabriella,
Gandini Carlo,
Kitsos Mikes,
Ponci Carolina
Publication year - 1993
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.530050810
Subject(s) - chemistry , enantiomer , nicardipine , bepridil , chiral column chromatography , selectivity , chromatography , acetonitrile , atenolol , high performance liquid chromatography , calcium , stereochemistry , organic chemistry , catalysis , verapamil , blood pressure , radiology , medicine
Three chiral calcium antagonist drugs, bepridil and two dihydropyridine derivatives (nicardipine and REC 15/2375), have been successfully separated within short retention times using either the α 1 ‐acid glycoprotein chiral stationary phase (Chiral AGP) or the ovomucoid column (Ultron ES‐OVM). Aqueous buffer at defined pH is modified by the addition of an organic component (propan‐2‐ol, acetonitrile, ethanol) in order to modulate the retention properties of each system. The influence of pH and percentage of organic modifier on retention, selectivity, resolution, and column performance are discussed for bepridil analyzed on Chiral AGP and for the two dihydropyridines (nicardipine and REC 15/2375) analyzed on Ultron ES‐OVM stationary phases. © 1993 Wiley‐Liss, Inc.